人血中三硝基甲苯血红蛋白加合物的结构鉴定和定量分析

Identification and determination of hemoglobin adduct in human occupational exposure to rinitrotoluene (TNT)

建立高效液相色谱定量测定三硝基甲苯（ＴＮＴ）血红蛋白（Ｈｂ）加合物的方法，并用于分析人的Ｈｂ加合物。结果表明，ＴＮＴ在体外可与人Ｈｂ反应形成加合物，并发现至少有两种类型：酸不稳定型和稳定型。前者占所有ＴＮＴ－Ｈｂ加合物的７１％。接触ＴＮＴ工人的Ｈｂ水解后，用液相色谱／质谱／质谱（ＬＣ／ＭＳ／ＭＳ）首次鉴定出了个４－氨基－２，６－二硝基甲苯（４Ａ），但未检测出在大鼠实验中所发现的２－氨基－４，６－二硝基甲苯（２Ａ）；接触组加合物浓度范围为０．０１４～０．７９２μｇ／ｇＨｂ。对照组未检出Ｈｂ加合物。

A HPLC quantitative assay was developed for the detection of TNT-hemoglobin (Hb) adducts, and was applied to detect adducts in human (in vivo and in vitro). The results showed that TNT can react with Hb in vitro and form TNT-Hb adducts. Two types of adducts at least were found: acid labileand persistent. The acid-labile adducts accounted for 71% of all TNT-Hb adducts in the human Hb following reaction with TNT in vitro. After hydrolysis of the Hb from exposed TNT workers, 4A was identified by LC/MS/MS,but 2A was not detected. The ranges of adduct in the exposed group was 0.014￣0.792μg/g Hb. In the control group, Hb-adducts were not detectable.