核黄素缺乏大鼠红细胞维生素E水平的变化及脂质过氧化关系的研究

Effect of riboflauin deficiency on eryihrocyie vitamin E level with relation to lipid peroxidation in rat

对两组大鼠分别喂饲核黄素缺乏（ＲＤ）膳和核黄素添加（Ｒ８，２２ｍｇ／ｋｇ饲料）膳８周后，测定了两组大鼠的红细胞维生素Ｅ（ＲＢＬＶｅ）、红细胞超氧化物歧化酶（ＳＯＤ）和红细胞丙二醛（ＭＤＡ）的水平。结果发现：ＲＤ组ＲＢＣＶｅ水平（４．７１７３±０．７７１０ｍｇ／ｇ蛋白质）显著低于ＲＳ组（５。３８６８±１．１５３７ｍｇ／ｇ蛋白质，Ｐ＜０．０５）。而ＲＤ组的ＲＢＣＳＯＤ（７７４５．２±６１０．１ｕ／ｇ蛋白质）和ＭＤＡ（０．６８６８±０．１３７２μｇ／ｇ蛋白质）则分别显著低于和高于ＲＳ组（８２６８．５±３０１．０ｎｕ／ｇ蛋白质，０．５５４８±０．０９８０，Ｐ＜０．０５）。研究提示，核黄素缺乏引起细胞膜脂质过氧化加重可能ＲＢＣＶｅ消耗增加。

Rat erythrocyte(RBC) Vitamin E (V_), erythrocyte superoxidant dismutase (SOD) and reythrocyte malonaldehyde (MDA) were measured after feeding a ribofiavin deficient (RD) diet or a ribofiavin supplemented (RS, 22 mg/kg) diet for 8 weeks. The results showed that RBC V_e level from RD rat (4.717 3=0.771 0 mg/g protein) was significantly lower than that from RS rat (5.386 8=1.153 7 mg/g protein, P<0.05),while the RBC SOD (7745.2±610.1 U/g protein) and MDA (0. 6868±0.137 2μg/g protein) from RD rat were significantiy lower and higher than that from RS rat (8268.5±301.0U/g proein and 0. 554±0.098 0μg/g protein, p<0.05) respectively. The study demonstrate that the snhanced lipid peroxidation resulted from ribofiavin deficiency may lead to an inorease in Vs consumption in RBC.