食品中诱惑红的测定方法

Method for determination of allura red in food

诱惑红是我国在１９９０年批准的一种食品着色剂。本文报道了汽水、糖果、糕点、冰淇淋等４种食品中诱惑红的测定方法。诱惑红的最大吸收波长５００ｎｍ；分离诱惑红与胭脂红、苋菜红、日落黄、柠檬黄、亮蓝的展开剂是（１）丁酮＋丙酮＋水＋氨水（７＋３＋３＋０．５）；（２）正丁醇＋无水乙醇＋１％氨水（６＋２＋３）；（３）２．５％柠檬酸钠＋氨水＋乙醇（８＋１＋２）。本方法加标回收率：糖果为９１．７６％～９５．８３％，汽水９０．４４％～９２．７３％，糕点８２．４８％～８４．９０％，冰淇淋７２．５％～８６．７０％。糖果、汽水、糕点、冰淇淋中最低检出浓度１０ｍｇ／ｋｇ（Ｌ），最低检出量为１μｇ。

Allura red is afood additive color which is approved to use in china in 1990. The method for detremination of Allura red in beverage,candy, cake and icecream was described. Allura red showed λmax 500nm. The moving phases for separation of Allura red and ponceau 4R, Amaranth, Sunset yellow, tartrazine, brilliant blue were:(1) butanone +acetone+water +ammonium hydroxide(7+3+3+0.5) (2)butanol+ethyl alcohol,absolute+1%ammonium hydroxide(6+ 2 + 3),(3)2.5%sodium citrate+ammonium hydroxide + ethylalcohol (8+ 1 +2) The recovery rates were 91. 76%～95. 83% in candy, 90. 44%～92. 73%in beverage,83. 48%～84. 90% in cake product,72.5～86. 7% in icecream. The detecting limit in the sample was 10mg/kg(L), The coefficient of variation was 5.07%.