摘要
建立了大鼠胃、肠中杀虫双和沙蚕毒素的气相色谱定量测定方法。胃和肠中杀虫双平均回收率分别为97.6%和116.2%,沙蚕毒素平均回收率分别为102.6%和99.8%。采用此方法观察经口灌胃杀虫双后在大鼠体内转化成沙蚕毒素的部位以及其转化的动态变化。TLC及GC定性、定量结果均表明胃是主要转化部位。给大鼠灌胃杀虫双(5.0mg/kg)1min后,胃中杀虫双总量(杀虫双及2.38倍的沙蚕毒素之和)与给药量相当,而此时约30%杀虫双己转化成沙蚕毒素,给药1h后,胃中未检出杀虫双和沙蚕毒素。杀虫双在胃中消失动态与时间呈良好线性关系。胃中杀虫双浓度与时间的关系为C=55.98e-0.25t+41.85e-0.
The methods of determination of shachongshuang and nereistoxin in stomach and intestine of rats were established. The average recoveries of Shachongshuang in stomach and intestine were 97.6%and 116.2%respectively and those of nereistoxin were 102.6% and 99.8% respectively.Using these methods,it was shown that the main organ in which Shachongshuang was transformed to nereistoxin was stomach. The toxicokinetic characteristcs following oral intubation with this pesticide(5mg/kg) indicated that after 1 minute the total amount of Shachongshuang(Shachongshuang plus 2. 38 times the amount of Nereistoxin) in the stomach was equal to the amount of intake,and about 30% of Shachongshuang was transformed to Nereistoxin.After 1 hour,neither Shachongshuang nor nereistoxin could be detected in the stomach.The disappearance of Shachongshuanng in stomach showed a linear relationship with time and could be represented by the following equation:C=55. 98e-0.25t+41. 85e-0.06t
出处
《卫生研究》
CAS
CSCD
北大核心
1994年第6期352-356,共5页
Journal of Hygiene Research
基金
国家自然科学基金