人LAK细胞对胃癌细胞的杀伤作用

KILLING FUNCTION OF LAK CELL AGAINST HUMAN STOMACH CARCINOMA CELLS

用人重组白细胞介素—２（ｒＩＬ－２）体外激活健康献血员外周血单个核细胞（ＰＢＭＣ），使之形成淋巴因子激活杀伤（ＬＡＫ）细胞，借助中性红摄入法，研究了ＬＡＫ细胞对胃癌细胞的杀伤作用机制。结果表明：①ＬＡＫ细胞对胃癌细胞具有明显杀伤作用，诱导４天后其活性达峰值；②ＩＬ一２对已诱导的ＬＡＫ细胞杀伤胃癌细胞活性无协同作用；③ＬＡＫ细胞上清液有明显杀瘤活性物质，而ｒＩＬ一２对胃癌细胞无明显毒性作用。提示在ＬＡＫ细胞诱导过程中，淋巴细胞可能自身分泌一些细胞毒因子。

eripheral blood monocytes from healthy donors were activated with human interleukin一 2toform lytnphokine一activated killer(LAK)cell in vitro. The mechanism of the killing function of LAKagainst human stomach carcinoma cells was investigated with neutral red incorporation method. Theresults showed:①LAK cells had significant cytotoxicity against stomach carcinoma cells.The activityreached the peak after 4 days' culture with l,000 u/ml of IL 2in CM.②IL一2didn’t work withthe activated LAK cells to kill human stomach carcinoma cells in vitro.③ LAK cell supernatants obvi-ously had a material for killing stomach carcinoms cells.These findings suggested that lymphocvtethemselves secret some cytotoxic factors in the process of foming LAK cells.