2型糖尿病患者大网膜与皮下脂肪组织抵抗素蛋白表达的研究

The expression of resistin protein in greater omentum and subcutaneous fat tissues in type 2 diabetic patients

目的 了解 2型糖尿病 (T2DM)患者大网膜与皮下脂肪组织抵抗素蛋白表达的情况,探讨肥胖和糖尿病间的关系。方法 Westernblot法检测大网膜及皮下脂肪组织抵抗素表达情况;测定空腹血胰岛素水平和血糖浓度,并计算胰岛素敏感指数;测量身高、体重、腰围、臀围、收缩压、舒张压,计算腰臀比、体重指数、体内脂肪百分比。结果 以OD值表示,正常对照组 ( 57411±1225. 59)大网膜与皮下脂肪组织抵抗素蛋白表达明显较单纯肥胖组 ( 41671±884. 38,P<0. 01 )、非肥胖T2DM组(42131±1286. 15,P<0. 01)、肥胖T2DM组(42677±1751. 10,P<0. 05)表达增高,后三组抵抗素蛋白表达无明显差异。四组自身的大网膜与皮下脂肪组织抵抗素蛋白表达无显著差异。逐步回归分析示腰臀比、体重指数、空腹血糖对抵抗素表达的作用显著。结论 正常对照组大网膜与皮下脂肪组织抵抗素蛋白表达明显较单纯肥胖组、非肥胖T2DM组、肥胖T2DM组表达增高,抵抗素与腰臀比、体重指数、空腹血糖呈显著负相关提示人体抵抗素可能不是肥胖和糖尿病的联系所在。

Objective To investigate the expression of resistin protein in greater omentum and subcutaneous fat tissue in type 2 diabetic patients and the relationship between obesity and diabetes mellitus.Methods The expression of resistin protein in greater omentum and subcutaneous fat tissues was measured using western blot method.Fasting insulin and glucose level were measured to calculate insulin sensitivity index.Height,weight,waist circumference,hip circumference,systolic blood pressure,diastolic blood pressure were measured to calculate waist-hip ratio,body mass index,body fat percentage.Results The expression of resistin protein in greater omentum and subcutaneous fat tissues in normal group(57411±1225.59)(arbitarary OD units) was more higher than that in obesity group(41671±884.38,P<0.01),type 2 diabetes mellitus without obesity group(42131±1286.15,P<0.01) and type 2 diabetes mellitus with obesity group(42677±1751.10,P<0.05)respectively,and there was no difference among obesity group,type 2 diabetes mellitus without obesity group and type 2 diabetes mellitus with obesity group.The expression of resistin protein in greater omentum fat tissue was compared with that in subcutaneous fat tissue in oneself and no difference was found.Stepwise regression analysis showed that expression of resistin protein was negatively correlated with WHR,BMI as well as FBG.Conclusion The expression of resistin protein in greater omentum and subcutaneous fat tissues in normal group was more higher than that in obesity group,type 2 diabetes mellitus without obesity group and type 2 diabetes mellitus with obesity group respectively.The negative correlation between expression of resistin and WHR,BMI,as well as FBG suggests that resistin may not links obesity and diabetes mellitus.