α－石英表面自由基和离体肺泡巨噬细胞内游离Ca^（2＋）、Mg^（2＋）浓度的关系

RELATIONSHIP BETWEEN CYTOPLASM FREE Ca￣2+, Mg￣2+ CONCENTRATION AND THE FREE RADICALS ON α-QUARTZ SURFACE

本文用ＡＲ－ＣＭ－ＭＩＣ阳离子测定系统及Ｆｕｒａ－２荧光技术，研究了研磨后表面存在自由基的α－石英，和离体肺泡巨噬细胞作用后细胞内游离Ｃａ２＋浓度（［Ｃａ２＋］）和游离Ｍｇ２＋浓度（［Ｍｇ２＋］ｉ）的关系．发现在含Ｃａ２＋和Ｍｇ２＋的介质中，α－石英表面自由基越多，［Ｃａ２＋］ｉ和［Ｍｇ２＋］ｉ升高越大；在无Ｃａ２＋和Ｍｇ２＋的介质中，新研磨的α－石英能引起［Ｃａ２＋］ｉ和［Ｍｇ２＋］ｉ升高．Ｃａ２＋和Ｍｇ２＋的升高可能部分由于细胞内结合钙和结合镁的释放．

In this paper, cytoplasm free Ca2+ and Mg2+ concentration ([Ca2+]i, [Mg2+]i) of alveolar macrophages in rabbit lung after incubation with α-quartz were determined. The relationship between [Ca2+]i, [Mg2+]i and the free radical intensity on α-quartz surface had been studied. The results showed that in Hank's medium the stronger the free radicals, the higher the [Ca2+]i and [Mg2+]i. In D-Hank's medium the [Ca2+]i and [Mg2+]i can be risen by the freshly ground α-quartz.