小鼠动情周期与超排效果

Estrous Cycle and Superovulation Effect in Mice

通过对25只小鼠用两种阴道涂片方法进行动情周期的观察,筛选出最佳涂片方法观察到的具有明显动情周期变化的小鼠进行超数排卵。根据不同生理周期将小鼠分组,分别为发情期(第1组)、发情后期(第2组)及间情期(第3组),而13只未知周期的小鼠作为对照组,比较各组间超排效果。结果表明:(1)用方法2涂片效果最好,方法2较方法1各时期阴道上皮细胞变化明显,对小鼠正常的生理周期影响相对较小,可观察到小鼠正常动情周期的变化规律。(2)不同生理周期小鼠的超排效果,以间情期可用胚百分率和平均每只小鼠获得胚胎数极显著高于发情期、发情后期及未知周期。

The experiment observed the estrous cycle of twenty-five mice with two vagina smear methods .The mice whose estrous cycle were regular were cheese to handle with superovulation method. According to the different periods (estrus,post-estrus,interestrus ),these mice classified into three test groups, and then other thirteen mice that were unknown estrous cycle were contrast group. The results showed that : (1) The method 2 was better than method 1, the method 2 had little effect to the estrous cycle of the mice. (2) The superovulation results of the interestrus mice were the best of all other periods. The rates of the valuable embryos and embryos quantity were significantly better than the estrus,post-estrus and unknown estrous cycle.