不同化学激活方法对绵羊孤雌生殖胚体外发育的影响

Parthogenic Development of Sheep Ooytes Activated by Different Chemical Methods

本研究利用常规化学激活剂离子酶素(Ionomycin)和乙醇分别与6-二甲基氨基嘌呤(6-Dimethylaminoputine,6-DMAP)和细胞松弛素B(CB)的不同组合对绵羊卵母细胞的激活后体外发育效果进行了比较。结果表明:在桑囊胚率上,Ionomycin联合6-DMAP与联合6-DMAP+CB差异不显著(40.4%vs46.0%),而与联合CB组差异显著(40.4%、46.0%vs 29.5%,p<0.05),与单独Ionomycin激活差异极显著(40.4%、46.0%vs 15%,p<0.01);乙醇联合6-DMAP+CB与联合6-DMAP、CB差异显著(46.9%vs 34.2%、36.2%,p<0.05),而与单独乙醇激活差异极显著(46.9%vs 21%,p<0.01)。

Activation is a key step for somatic cell cloning and micro-fertilization. The objective of present study were to compare parthenogenic development of sheep oocytes activated by different sequential treatments with either 7% ethanol or 5mM ionomycin, and either 2mM 6-dimethylaminopurine (6-DMAP) or 6-DMAP+ 5μg/mL cytochalasin B(CB).The results show that: 1)There was no significant difference in morula and blastocyst development rate between ionomycin -6-DMAP and ionomycin-6-DMAP+CB sequential treatments(40.4%VS46.0%), but the two treatments had a higher rate of morula and blastocyst development rate (40.4%?46.0% VS 29.5%, p<0.05) compared to ionomycin-CB treatment, and were significantly higher than only ionomycin treatment(40.4%?46.0% VS 15%, p<0.01);2) ethanol-6-DMAP+CB had a higher rate of morula and blastocyst development rate than ethanol-6-DMAP and ethanol- CB(46.9% VS 34.2%?36.2%,p<0.05), and it was significantly higher than only ethanol treatment(46.9% VS 21%,p<0.01).