丛枝菌根对枳实生苗抗旱性的影响研究

Effects of arbuscular mycorrhiza on drought tolerance of Poncirus trifoliata

在自然水分干旱胁迫和胁迫解除复水条件下 ,研究了丛枝菌根对 1年生枳实生苗生长和抗旱性的影响 .结果表明 ,接种丛枝菌根真菌Glomusmosseae 93显著增加枳实生苗的株高、茎粗和鲜重 ,提高了幼苗移栽成活率 .在自然水分干旱胁迫和胁迫解除复水过程中 ,丛枝菌根提高或者极显提高了叶片可溶性糖含量、叶片和根系的可溶性蛋白质含量、超氧化物歧化酶活性、过氧化物酶活性和过氧化氢酶活性 ,从而提高了枳实生苗的渗透调节能力 ,增强了其保护系统能力 ,降低了细胞膜脂过氧化 ,使枳实生苗抗旱能力增强 .表明丛枝菌根真菌增强寄主植物抗旱能力的作用机制与保护系统相关 .

This paper evaluated the effects of arbuscular mycorrhizal colonization on the growth and drought tolerance of Poncirus trifoliata in a potted culture under natural water stress and resuming water supply.The results showed that the arbuscular mycorrhizal colonization by Glomus mosseae 93 could significantly increase the plant height,stem diameter,and fresh weight of myocrrhizal plant.By the end of the experiment,the survival percentage of transplanted mycorrhizal plant was 7.1% higher than that of nonmyocrrhizal plant.During natural water stress and resuming water supply,the soluble sugar and soluble protein contents and the SOD,POD and CAT activities of root or leaf were higher or significantly higher in mycorrhizal than in nonmycorrhizal plant,suggesting that arbuscular mycorrhizal colonization could improve the osmoregulatory response of myocrrhizal plant,enhance its protective capability,and depress its cell membrane oxidative damage to lipids.Consequently,the drought tolerance of inoculated Poncirus trifoliata was enhanced.It was considered that the functional mechanism of arbuscular mycorrhizal fungus in enhancing the drought tolerance of host plant was closely related to the protective systems such as SOD,POD,CAT,and soluble protein,etc.