拮抗木霉菌株T21发酵条件的研究

Studies on Fermentation Condition of Trichoderma Strain "T21"

研究了拮抗木霉菌株T2 1的发酵条件 ,旨在用于木霉生防制剂的工业化生产。结果表明 :最适T2 1菌丝生长的发酵培养基为①、②和⑥号培养基 ;碳、氮源分别为蔗糖和蛋白胨时 ,T2 1的生物量最高。以⑥号培养基为发酵培养基 ,在装瓶量 5 0ml、接种量 6%、初始pH值 5～ 7、温度 2 5℃、摇床转速 180r/min的条件下 ,培养 3～ 4d时 ,发酵液中的T2 1生物量最高。

The intention of this experiment was to research the fermentation condition of Trichoderma Strain'T21' and find out the qualification that could be used to ferment the mycelial . Then we can use the condition to produce the biocontrol preparation. Synthesizing all the results,we got the best fermentation condition: medium No 1,2 and midium No 6 was the best medium, the chitinase activity was tiptop when sucrose as carbon source, and peptone as nitrogen source.50 ml medium No 6 volume in 250 ml triangle flask bulk,6% inoculating volume, initializing medium pH to 5～7,25℃,180 r/min to ferment for 3~4 days. The hyphae quantity reached to the highest level.