恩诺沙星在眼斑拟石首鱼体内的药物代谢动力学

Pharmacokinetics of Enrofloxacin in Sciaenops ocellatus

应用反相高效液相色谱法 (RP- HPL C)研究了恩诺沙星在眼斑拟石首鱼 (Sciaenops ocellatus)体内的药物代谢动力学。实验数据经 DAS药代动力学分析软件分析后得出 :腹腔注射组血浆的药时数据符合一级吸收二室模型 ,动力学方程为 :C =4 .92 5 e- 1 .4 52 t +2 .730 e- 0 .0 75t ,其主要药代动力学参数 :AU C37.5 33mg· L- 1· h、Cmax 4 .74 7mg/ L、Tmax0 .75 0 h、t α0 .4 77h、t β9.2 92 h、Vd/ F 1 .6 76 L / kg、t Ka0 .1 70 h、Ka 4 .0 81 / h、K 6 .71 4 / h、K1 0 0 .1 91 / h、K1 2 0 .76 9/h、K2 1 0 .5 6 6 / h;灌服组血浆的药时数据符合一级吸收一室模型 ,动力学方程为 :C =6 .4 82 (e- 7.0 92 t- e- 1 0 .356 t) ,其主要药代动力学参数 :AU C1 5 .80 5 mg· L- 1 · h、Cmax2 .770 mg/ L、Tmax1 .5 0 0 h、t α4 .989h、Vd/ F2 .0 72 L/ kg、Ka7.0 92 /h、K 1 0 .35 6 / h。结果表明 ,腹腔注射给药比灌服给药吸收快 ,血药浓度达峰时间短于灌服给药 ,但血药浓度峰值明显高于灌服给药 (P<0 .0 5 ) ,血浆中恩诺沙星的回收率为 94 .5 79%。

The pharmacokinetics of enrofloxacin(EF) in the Sciaenops ocellatus with peritoneal injection and oral gavage administration(5 mg/kg) were investigated using the RP-HPLC in this study.The plasma EF concentration-time data were accorded with a two-compartment model with first-order absorption after peritoneal injection,the pharmacokinetic equation:C=4.925e^(-1.452t)+2.730e^(-0.075t),the main parameters were as follows:AUC 37.533 mg·L^(-1)·h,C_(max) 4.747 mg/L,T_(max) 0.750 h,t_(1/2α) 0.477 h,t_(1/2β) 9.292 h,Vd/F 1.676 L/kg,t_(1/2Ka) 0.170 h,Ka 4.081/h,K 6.714/h,K_(10) 0.191/h,K_(12) 0.769/h,K_(21) 0.566/h.The EF concentration-time data were fitted to a one-compartment model with first-order absorption after oral gavage administration,the pharmacokinetic equation:C=6.482(e^(-7.092t)-e^(-10.356t)),the main parameters were followed:AUC 15.805 mg·L^(-1)·h,C_(max) 2.770 mg/L,T_(max) 1.500 h,t_(1/2α) 4.989 h,Vd/F 2.072 L/kg,Ka 7.092/h,K 10.356/h.These results indicated that peritoneal injection led to more rapid absorption,higher plasma EF concentration and less retaining time than oral gavage administration,Recovery of EF extracted from plasma by 1% acetic acid in ethanol was 94.579%.