诺氟沙星荷移络合物的荧光特性和应用

The Fluorescence Characteristics of Charge Transfer Complexes of Norfloxacin and Their Application

研究了电子受体四氯对苯醌(TCBQ)和2,3-二氰-5,6-二氯-1,4-对苯醌(DDQ)与电子给体诺氟沙星之间的荷移反应.实验发现,诺氟沙星与以上两种电子受体可生成稳定的n-π络合物,其荧光强度较之诺氟沙星分别增强了12 和17倍.据此建立了两种基于荷移反应简便可靠地测定诺氟沙星的荧光光谱新方法.TCBQ法和DDQ法测定诺氟沙星的浓度分别为0.02 ～1.2和0.14～2.8 mg/L时, 荧光强度与质量浓度呈良好的线性关系.该方法已成功地用于药物制剂中诺氟沙星含量的测定, 其回收率(%)分别为98.87±1.42～99.63±1.26和98.49±1.46～99.21±1.62.本方法也可用于生物体液中诺氟沙星的测定.

The-charge-transfer reaction between tetrachloro-p-benzoquinone (TCBQ), 2,3-dichloro-5,6-dicyano-1,4-benzoquinore (DDQ) as a pi-electron acceptor and norfloxacin (NOR) as an electron donor has been studied by spectrofluorimetry. TCBQ and DDQ were found to react with the drugs toproduce stable n-pi complexes and the fluorescence intensity of the complexes was enhanced in 12 and 17 fold higher than that of the studied drugs itself, respectively. Thus two simple and reliable fluorescence spectrometric methods for the determination of NOR have been developed based on the charge transfer reaction. The linear calibration graphs was obtained over the NOR concentration range of 0. 02 similar to 1. 2 and 0. 14 similar to 2. 8 mg/L with TCBQ and DDQ, respectively. The proposed procedures could be applied successfully for the determination of the investigated drugs in their pharmaceutical preparations with good recovery percent ranged ( %) from 98. 87 +/- 1. 42 to 99. 63 +/- 1. 26 in the TCBQ method, 98. 49 +/- 1. 46 to 99. 21 +/- 1. 62 in the DDQ method. They were also applied for the determination of norfloxacin in urine samples.