RP-HPLC法同时测定延胡索药材中延胡索乙素和原阿片碱

RP-HPLC simultaneous determination of tetrahydropalmatine and protopine in Rhizoma Corydalis

建立了反相高效液相色谱(RP HPLC)法同时测定延胡索药材中延胡索乙素与原阿片碱含量的方法,并对不同产地延胡索药材中延胡索乙素与原阿片碱含量进行了考察.结果表明,使用PhenomenexLunaC18色谱柱,甲醇 磷酸盐缓冲溶液(pH=7 68,V∶V=65∶35)流动相,在检测波长281nm下,测定延胡索乙素与原阿片碱的线性范围分别为0 13～4 68μg和0 09～3 24μg,平均回收率分别为101 86%和98 13%,RSD分别为1 42%和1 36%.结果表明,此法简便、准确、快速,可用于药材中延胡索乙素与原阿片碱含量的测定,也可用于含这两种生物碱的药材的质量评价;两种生物碱含量在不同产地及来源延胡索药材中有较大差别.

A reserved-phase HPLC method is established for simultaneous determination of the content of tetrahydropalmatine and protopine in Rhizoma Corydalis from different habitats. The samples are separated on a Phenomenex Luna C_(18) column with mobile phase methanol-phosphate buffer (pH 768, 65∶35) and detected at wavelength of 281 nm. The average recoveries respectively for tetrahydropalmatine and protopine are 10167% and 9813%, RSD are 144% and 137%. The contents of the two alkaloid in Rhizoma Corydalis from different habitats show obvious difference. The method is simple, accurate, quick and suitable to determine content and evaluate quality for the drugs.