被动吸烟致金黄地鼠神经管上皮细胞的过度凋亡

Excessive Apoptosis in Neuroepithelium Induced by Passive Smoking

目的探讨被动吸烟对神经管的致畸作用与细胞凋亡的关系。方法采用核固红结晶紫染色方法,原位末端标记法及形态计量方法定量分析了被动吸烟致金黄地鼠神经管上皮细胞凋亡的变化。结果被动吸烟引起神经管上皮细胞的过度凋亡,实验各组均较对照组神经管上皮细胞凋亡显著增加,而细胞分裂相减少孕9d时,神经管上皮细胞的凋亡细胞最多,凋亡指数为9.82±3.67,与对照组相比有统计学意义(P<0.01);孕10d时细胞凋亡逐渐减少;孕11d后,细胞凋亡降至最低,凋亡指数为5.21±2.82,与对照组相比有统计学意义(P<0.05)。结论被动吸烟可以促使神经管上皮细胞的过度凋亡,可能是导致神经管畸形发生的重要原因之一。

Objective To investigate the apoptosis in neural epithelium induced by passive smoking. Methods By using the nuclear fast red-crystal violet staining method and situ TUNEL technology, the quantitative changes of apoptosis in neural epithelium in gold hamster were analyzed. Results Excessive apoptosis and less cell division were observed in neural epithelium compared with the control group. On 9th days of pregnancy, there were most apoptosis cells, the apoptosis index was 9.82 ±3.35 which is statically significant compared with control group (P<0.01), after 10 days of pregnancy, the observed apoptosis cells decreased gradually and to the least on the 11 th days of pregnancy. Conclusion Passive smoking can cause excessive apoptosis in neural epithelium, which may be one of the important ways by which the deformation of neural tube will occur.