毛冬青甲素的核磁共振法分析

Structural Analysis of Ilexonin A by NMR

采用1H、13C、DEPT(无畸变极化转移增益法)、1H-1H COSY(氢-氢化学位移相关谱)、HMQC(异核多量子相关谱)、HMBC(异核多键相关谱)、NOESY(二维核欧沃豪斯效应谱)等多种NMR分析方法, 对毛冬青甲素的1H和13C NMR谱信号进行了归属; 并对生产过程常同时存在的丁二酸二钠也进行了谱线归属, 提出通过1H NMR法对产品中的丁二酸二钠进行定量测定。为其结构鉴定和生产过程中的质量控制提供了重要依据。

The molecular structure of ilexonin A,a blood platelet aggregating agent,was elucidated by1D NMR and2D NMR techniques( 1 H, 13 C,DEPT,gCOSY,gHMQC and gHMBC).The 1 H and 13 C NMR sigˉnals for ilexonin and succinic acid disodium salt existing simultaneously in the course of production were asˉsigned.A quantitative method for the determination of succinic acid disodium salt in ilexonin A using 1 H NMR technique was developed.The data obtained could be used for its quality control and structure identificaˉtion in the course of production.