PBMC中HBV cccDNA与HBV宫内感染关系的研究

STUDY ON THE RELATIONSHIP OF PBMC HBV cccDNA AND INTRAUTERI NE INFECTION.

目的 :对外周血单个核细胞 (peripheralbloodmononuclearcells,PBMC)中乙型肝炎病毒 (HBV)共价闭合环状DNA (covalentlyclosedcircularDNA ,cccDNA)进行扩增 ,并对其产物直接测序鉴定 ,以证实PBMC为HBV肝外复制的场所 ,从而进一步证实PBMC感染为乙型肝炎宫内感染的途径之一。方法 :选择性PCR扩增PBMC中HBVcccDNA ,并对 1例阳性产物直接测序鉴定 ,测序结果与GeneBank中已知序列进行同源性比较。结果 :HBsAg阳性孕妇PBMCHBVcccDNA的检出率为 9 .93% ,新生儿PBMCHBVcccDNA的检出率为 4 . 5 8% ,PBMCHBVcccDNA与公布的已知序列的同源性为 10 0 %。结论 :PBMC为HBV肝外复制的场所 ,PBMC感染很可能是乙肝宫内感染的传播机制之一。

Objective:To confirm that PBMC is the replic ating location of HBV DNA outside the liver and that the infection of PBMC is on e of the HBV i ntrauterine infecting routes by amplifying the HBV cccDNA of PBMC and confirming its sequence.Method:Selective PCR was used to amplify the HBV cc cDNA of PBMC and the HBV ccc DNA was directly sequenced with PCR products. The sequenced res u lt was compared with data in GeneBank.Results:The detection rate of PBMC HBV cccDNA is respe ctively 9.93%in pregnanting women with positive HBsAg and 4.58% in their newbo rns. The sequence of the HBV cccDNA of PBMC showed 100% identity to the reported sequence. Conclusion:PBMC is the replicating location of HBV DNA outsi de the liver and the infection of PBMC is possibly one of the HBV intrauterine i nfecting routes.