支气管哮喘患者血清白介素-10与内皮素-1变化的研究

Study of IL-10 and ET-1 Levels in the Serum of Patients with Asthma

目的 :探讨支气管哮喘患者不同时期血清白介素 (IL) - 10、内皮素 (ET) - 1及IgE水平的变化及临床意义。方法 :采用酶联免疫法及放射免疫法分别测定不同时期支气管哮喘患者血清IL - 10、IgE和ET - 1水平 ,并与正常对照组进行统计分析。结果 :支气管哮喘患者发作期血清IL - 10水平显著低于缓解期和正常对照组 (P <0. 0 1) ,而ET - 1和IgE水平却显著高于缓解期和正常对照组 (P <0 .0 1)。IL - 10与ET - 1、IgE之间存在负相关 (r=- 0 . 6 8,r=- 0 . 74 ,P <0 . 0 1) ,而ET - 1与IgE之间呈正相关 (r=0 6 6 ,P <0 .0 1)。结论 :IL -10和ET - 1参与支气管哮喘发病的整个过程 ,在支气管哮喘患者不同时期检测血清IL - 10和ET - 1水平具有重要的临床意义。

Objective:To explore the changes of levels of serum IL-10,ET-1 and IgE of the patients with asthma at stage of episode or remission and their clinical significance. Methods:The serum levels of IL-10 and IgE were measured in 35 cases of asthma at stage of episode and 31 cases of asthma at stage of remission and 30 healthy controls by ELISA, the serum levels of ET-1 were measured by radioimmunoassay method. Results:The level of IL-10 was (11.8±4.4)pg/ml in the asthma attacking patients, which was significantly lower than those of remission patients (15.9±6.0)pg/ml and normal controls (19.2±7.3)pg/ml(P<0.01). The levels of ET-1 and IgE in the asthma attacking patients were (78.4±8.3)ng/L and (364.2±105.7)IU/ml, respectively, significantly higher than those of remission patients (39.2±7.8)ng/L, (219.7±41.6)IU/ml and normal controls(34.7±6.5)ng/L, (89.4±26.5)IU/ml(P<0.01). It was suggested that ET-1 and IgE had negative correlation with IL-10 (r=-0.68,r=-0.74, P<0.01),and ET-1 had positive correlation with IgE(r=0.66, P<0.01).Conclusion:IL-10 and ET-1 participate in the occurrence and development of asthma. The serum level of IL-10 and IgE in asthma is significant.