发酵条件对发酵产氢细菌B49产氢的影响

EFFECTS OF FERMENTATION CONDITIONS ON HYDROGEN PRODUCTION BY FERMENTATIVE HYDROGEN-PRODUCING BACTERIAL STRAIN B49

采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是B49生长、产氢的适宜氮源;葡萄糖是B49发酵产氢的最适宜底物,当浓度为10g/L时,B49的葡萄糖利用率为100%,氢气得率为1.69mol H_2/mol glucose;此外,B49可利用小麦、大豆、玉米、土豆及糖蜜废水和啤酒废水产氢,其中利用糖蜜废水、啤酒废水产氢分别为137.9ml H_2/ g COD和49.9ml H_2/g COD。

The effects of glucose concentration, inoculation volume, temperature, nitrogen source and various organic substrates on hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49 (AF481148 in EMBL) were investigated in batch fermentation experiments. The inoculation volume could affect hydrogen production. The favorable temperature for cell growth and hydrogen production is 35°C. B49 could produce hydrogen using organic nitrogen source only. Glucose is the best substrate producing hydrogen for B49. Glucose consumption and hydrogen yield with 10 g/L glucose are 100% and 1.69 mol H2/mol glucose, respectively. In addition, B49 could generate hydrogen from other organic substrates such as wheat, soybean, corn, potato, molasses and brewage wastewater. Hydrogen yields from molasses wastewater and brewage wastewater were 137.9 ml H2/g COD and 49.9 ml H2/g COD, respectively.