用基因枪法将抗除草剂bar基因导入小麦的研究

Studies on transferring herbicide resistance bar gene into wheat by particle bombardment

用基因枪法将含有抗除草剂bar基因的植物表达载体导入优良品种小麦幼胚愈伤组织, 并通过GUS瞬时表达对沉淀剂、轰击距离、金粉用量等轰击参数进行了优化。用除草剂对轰击后的材料进行分化生根筛选, 共获得 33株再生苗。通过PCR检测和涂叶检测, 初步证明其中 4株已将bar基因整合到小麦基因组中, 平均转化率为 0 33%。

The plant expression vector carrying the herbicide resistance bar gene was introduced into wheat immature embryo calli using particle bombardment.Parameters of bombardment were optimized by GUS transient expression,such as precipitants,distance,and amount of gold powder.After selection on media supplemented with different concentration of PPT,33 resistance plants were regenerated.It was demonstrated that the bar gene had been intergraded into 4 plants genome and expressed in transgenic plants by PCR analysis and spaying herbicide on the leaf.The average transformation frequency is about 0.33%.