富硒麦芽对黄曲霉毒素B_1致肝肿瘤大鼠抗氧化作用及γ-GT酶活性的影响

Effect of selenium-enriched malt on the antioxidative function and γ-GTase activity in the course of hepatocarcinogenesis induced by aflatoxin B_1(AFB_1)in rats

刚断奶SD大鼠60只,体重50～60 g,随机均分成4组,每组雌性7只,雄性8只.在组Ⅰ(正常对照组)、组Ⅱ(试验对照组)和组Ⅲ(高亚硒酸钠组)的基础饲料中分别添加亚硒酸钠,使饲料硒含量分别为0.1、0.1和0.3 mg·kg-1;组Ⅳ(富硒麦芽组)在基础饲料中添加富硒麦芽,使硒含量达0.3 mg·kg-1.各组饲喂基础料,至体重增长到150～200 g(第32天)时,除组Ⅰ外,其余3组大鼠实施'黄曲霉毒素B1(aflatoxin B1,AFB1)致肝癌短期实验模型程序',并测定肝、肾组织和血中硒含量及谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和γ-GT酶活性、丙二醛(MDA)和一氧化氮(NO)含量.实验结果显示:富硒麦芽较亚硒酸钠能更明显地提升全血和组织中GSH-Px活性,提高SOD水平,有效清除肾脏MDA,减少γ-GT灶.表明:富硒麦芽比亚硒酸钠能更有效地提高大鼠抗氧化能力,抑制肝细胞增生,从而抵抗AFB1所致肝肿瘤.

Sixty SD rats were randomly divided into 4 groups of 15 rats each,8 male and 7 female.The selenium contents in feed of group Ⅰ,Ⅱ and Ⅲ were 0.1,0.1and 0.3 mg·kg -1 from sodium selenite.0.3 mg·kg -1 selenium from Se-enriched malt was supplemented to the diet of group Ⅳ.After 32 days,rats of group Ⅱ,Ⅲ and Ⅳ were treated with the program“short-term animal hepatocarcinoma model induced by aflatoxin B 1(AFB 1)”.The selenium content,γ-GTase activity and antioxidative indexes,named as glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)and malonadehyde(MDA)in blood and tissue were measured.The data showed that effect of selenium from Se-enriched malt excelled sodium selenite in the aspects of raising GSH-Px and SOD activity,clearance of kidney MDA and decreasing the pathological proliferation of hepatocyte.In conclusion,the effect of selenium from by stimulating the antioxidative function and suppressing the pathological proliferation of hepatocyte Se-enriched malt on the hepatocarcinogenesis induced by AFB 1 was better than that of sodium selenite.