紫甘蓝中氨基甲酸酯类农药多残留分析方法研究

Analysis on residues of carbamate pesticides in Brassica oleracea L.

建立了高效液相色谱柱后衍生法测定紫甘蓝中9种氨基甲酸酯类农药及2种代谢物残留量的方法.采用C18、PSA小柱固相萃取分离净化,用ODS柱(柱槽温度50 ℃)在梯度淋洗条件下对9种氨基甲酸酯类农药及2种代谢物进行分离,通过柱后衍生化,荧光检测器(荧光波长为445 nm)定性定量测定紫甘蓝中9种氨基甲酸酯类农药及2种代谢物(杀线威、灭多威、涕灭威、恶虫威、甲萘威、硫苯威、硫双灭多威、仲丁威、甲硫威、甲硫威代谢物1、甲硫威代谢物2)的残留量.结果表明, 9种农药及2种代谢物在28 min内可以得到很好的分离,线性范围为0.01～50 mg·L-1,线性相关系数R2≥0.998 0,检出限为0.70～5.0 μg·L-1,方法回收率为80.6%～103.3%.

Residues of 11 carbamates(oxamyl,methomyl,methiocab-sulfoxide,methiocarb-sulfon,aldicarb,bendiocarb,carbaryl,ethiofencarb,thiodicarb,fenobucarb,methiocarb)in purple cabbage were determined using C 18 and PSA solid-phase extraction and high performance liquid chromatography with post-column derivatization reaction and fluorescence detection.The 11 carbamates could be well separated within 28 min.The correlation coefficients(R 2)of this method were more than 0.998 0,and their calibration curves were linear in the range of 0.0150 mg·L -1 .The recoveries of pesticides ranged from 80.6%103.3%,with detection limits being 0.705.0 μg·L -1 .