肺结核与氧化应激关系的初步探讨

The correlation between pulmonary tuberculosis and oxidative stress

目的探讨肺结核与氧化应激的关系及其可能的机制。方法用分光光度分析法检测50例肺结核患者(PPT)和50例健康成人志愿者(HAV)的血浆维生素C(VC)、维生素E(VE)、β-胡萝卜素(β-CAR)含量和红细胞丙二醛(MDA)含量以及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性。结果与HAV者比较,PPT患者的MDA平均值显著增高(P<0.001);而VC、VE、β-CAR、SOD和CAT均显著降低(均P<0.001)。控制年龄的偏相关分析提示,随着PPT病程的延长,MDA值逐渐增高(r=0.4255,P<0.01),而VC、VE、β-CAR、SOD和CAT值逐渐降低(r分别为-0.3288、-0.3816、-0.4744、-0.3169和-0.3768,P<0.05～0.01)。结论肺结核患者体内的氧化应激病理性加剧,且与病程有关。

Objective To investigate the correlation between pulmonary tuberculosis and oxidative stress, and to explore its potential mechanism. Methods The contents of vitamin C (VC), vitamin E (VE) and β-carotene (β-CAR) in plasma and the contentsof malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase (CAT) in erythrocytes of 50 patients with pulmonary tuberculosis (PPT) and 50 healthy adult volunteers (HAV) were determined by spectrophotometry. Results Compared with the HAV group, the average value of MDA in the PPT group was significantly increased (P<0.001), while VC, VE, β-CAR, SOD and CAT in the PPT group were significantly decreased (P<0.001). The partial correlation analysis controlled with age suggested that with prolonged the course of disease, the value of MDA in the PTT group was gradually increased (r=0.4255, P<0.01), while VC, VE, β-CAR, SOD and CAT were gradually decreased (r=-0.3288, -0.3816, -0.4744, -0.3169 and -0.3768,respectively, P<0.05-0.01). Conclusions The present study suggests that there is a pathologically aggravated oxidative stress in patients with pulmonary tuberculosis, and such oxidative stress is related to the course of disease.