摘要
目的探讨血管紧张素转换酶抑制剂(ACEI)苯那普利单用及与血管紧张素II-1型受体拮抗剂(ARB)伊贝沙坦合用,对自发性高血压大鼠(SHR)血压及心肌、肾皮质转化生长因子β3(TGFβ3)mRNA表达的影响。方法选用12周龄SHR24只,随机分苯那普利组、苯那普利与伊贝沙坦联合干预组、SHR组,每组8只。采用灌胃法分别给苯那普利组大鼠灌入苯那普利(8mg·kg·d-1),联合干预组大鼠灌入苯那普利(8mg·kg-1·d-1)与伊贝沙坦(40mg·kg-1·d-1)混合液;SHR和WKY对照组灌入自来水,共8周。观察药物对心率、血压及处死后左心室心肌和肾皮质TGFβ3mRNA表达水平的影响。结果与SHR对照组比较,苯那普利组、苯那普利与伊贝沙坦联合干预组大鼠,其心肌和肾皮质TGFβ3mRNA表达和血压水平较SHR组大鼠显著降低(P<0.01);而与苯那普利组大鼠比较,联合干预组大鼠心肌、肾皮质TGFβ3mRNA表达没有显著降低(P>0.05)。结论ACEI苯那普利单用和ARB与ACEI合用均能显著降低SHR的血压及抑制心肌、肾皮质TGFβ3的mRNA表达,这可能是防治高血压心、肾靶器官损害的机制之一。
Objective To investigate the effect of ACE inhibitor Benazepril alone or combining with AT1 receptor antagonist irbesartan(ARB) on transforming growth factor beta 3 (TGFβ3) gene expression in myocardium and renal cortex of spontaneously hypertensive rats (SHR). Methods Twenty-four 12-week SHR were randomly divided into three groups including a positive control group treated without medicine and two active groups treated with Benazepril (8mg·kg-1·d-1) alone and Benazepril (8mg·kg-1·d-1) combining with irbesartan (40mg·kg-1·d-1). After 8 weeks, blood pressure was measured by tail-sleeve method. TGFβ3 mRNA expression in myocardium and renal cortex was detected by real-time quantitative RT-PCR method. Results Compared with positive group of SHR, gene expression of TGFβ3 and the levels of blood pressure were significantly decreased in myocardium and renal cortex of two active groups treated with benazepril alone or combining with irbesartan (P<0.01). However, TGFβ3 expression of the group treated with Benazepril combining with irbesartan was not further decreased compared with group treated with Benazepril alone (P<0.05). Conclusion Benazepril alone or combining with irbesartan could significantly down-regulated expression of TGFβ3 mRNA in myocardium and renal cortex of SHR, which may be one of the mechanisms in regressing the damage of target organs caused by hypertension.
出处
《浙江医学》
CAS
2005年第3期183-185,共3页
Zhejiang Medical Journal
