湖北八角莲属植物过氧化物酶同工酶分析

A STUDY ON THE PEROXIDASE ISOZYMES OF DYSOSMA FROM HUBEI PROVINCE

用聚丙烯酰胺凝胶电泳分离湖北八角莲属（Ｄｙｓｏｓｍａ）４种植物的过氧化物酶同工酶，并采用薄层扫描和排序分析法，对其亲缘关系进行了比较分析。结果表明，六角莲（Ｄ．ｐｌｅｉａｎｔｈａ）与八角莲（Ｄ．ｖｅｒｓｉｐｅｌｌｉｓ）、小八角莲（Ｄ．ｄｉｆｆｏｒｍｉｓ）、乌云伞（Ｄ．ｌｉｃｈｕａｎｅｎｓｉｓ）的亲缘关系较远，小八角莲（Ｄ．ｄｉｆｆｏｒｍｉｓ）、八角莲（Ｄ．ｖｅｒｓｉｐｅｌｌｉｓ）与乌云伞（Ｄ．ｌｉｃｈｕａｎｅｎｓｉｓ）亲缘关系较近，同时还为乌云伞（Ｄ．ｌｉｃｈｕａｎｅｎｓｉｓ）作为一个独立种提供了佐证。

The peroxidase isozymes of four species of Dysosma from Hubei Province were analyzed by using polycrylamide gel lectrophoresis and TLC-scanning.The phylogenetic relationships of the four species were discussed and the result supports the view of point of regarding D.lichuanensis as a new specles.