绿色荧光蛋白在视网膜疾病研究中的应用
摘要
从海洋无脊椎动物体内分离出的绿色荧光蛋白(GFP),是一种极具潜力的标记物。其 内源荧光基团在受到紫外光或蓝光激发时可高效发射清晰可见的绿光,且荧光性质稳定,与现有的标 记物相比,GFP具有无可比拟的优势。在视网膜疾病基因治疗的研究中,GFP具有广阔的应用前景。
出处
《国外医学(眼科学分册)》
2005年第1期59-62,共4页
Foreign Medical Sciences(Section of Ophthalmology )
参考文献19
-
1Shimomura O, Johnson FH, Saiga Y. Extraction, purification and properties of aequorin, a biolumlnescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol, 1962, 59; 223-239.
-
2Chalfie M, Tu Y, Euskirehen G, et al. Green fluorescent protein as a marker for gene expression. Science, 1994, 263:802-805.
-
3Prasher DC, Eckenrode VK, Ward WW, et al. Primary structure of the Aequorea victoria green-fluorescent protein. Gene, 1992, 111 :229-233.
-
4Yang F, Moss LG, Phillips GN Jr. The molecular structure of green fluorescent protein. Nat Bioteehnol, 1996, 14:1246-1251.
-
5Cody CW, Prasher DC, Westler WM, et al. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry, 1993, 32:1212-1218.
-
6Tsien RY. The green fluorescent protein. Annu Rev Biochem, 1998,67:509-544.
-
7Gubin AN, Reddy B, Njoroge JM, et al. Long-term, stable expression of green fluorescent protein in mammalian cells. Biochem Biophys Res Commun, 1997, 236: 347-350.
-
8Persons DA, Allay JA, Allay ER, et al. Retroviral-mediated transfer of the green fluorescent protein gene into murine hematopoietic cells facilitates scoring and selection of transduced progenitors in vitro and identification of genetically modified cells in vivo. Blood, 1997,90: 1777-1786.
-
9Dreyer EB, Vorwerk CK, Zurakowski D, et al. Infection with adeno-associated virus may protect against excitotoxicity. Neuroreport,1999, 10: 2887-2890.
-
10Coxon A, Bestor TH. Proteins that glow in green and blue. Chem Biol, 1995, 2: 119-121.
-
1魏捷,蒋华.绿色荧光蛋白特性及其在角膜内皮疾病研究中的应用[J].眼科研究,2009,27(7):624-627. 被引量:1
-
2王亚冬.眼表疾病研究进展[J].眼科新进展,2003,23(3):206-208. 被引量:3
-
3董微丽,邹留河.共焦显微镜检查在角膜病的应用[J].国外医学(眼科学分册),2003,27(4):249-253.
-
4《黄斑部疾病显微手术学》一书即将出版[J].中华眼底病杂志,2005,21(6):376-376.
-
5王骥,毛伟,文丰,周宏健,陆斌,张哲.超声角膜测厚仪与眼前节分析仪在飞秒激光辅助的角膜移植术后测量角膜中央厚度的对比研究[J].现代实用医学,2014,26(10):1252-1252. 被引量:1
-
6霍栎安.硬性透气性角膜接触镜在青少年儿童中的临床应用[J].医学综述,2007,13(16):1262-1265. 被引量:5
-
7徐巍,郭健,徐国兴.骨髓间充质干细胞在视网膜疾病研究中的应用[J].海峡科学,2010(5):45-47.
-
8胡文明,宋月莲,姚亦群,钱江,殷汝桂,郭文龙,蔡林生,徐振兴,缪士文.透明质酸钠与甲基纤维素用于白内障人工晶体植入术中临床观察[J].中国实用眼科杂志,1994,12(9):528-529. 被引量:10
-
9刘肖艺,刘庆淮,陈曦,程海霞.玻璃体腔注射抗血管内皮生长因子单克隆抗体bevacizumab治疗视网膜分支静脉阻塞伴黄斑水肿的疗效观察[J].中华眼底病杂志,2012,28(5):515-517. 被引量:2
-
10AREDSResearchGroup 陆遥.眼部疾病与高剂量抗氧化剂和锌治疗年龄相关性眼科疾病患者死亡率之间相关性的研究[J].美国医学会眼科杂志(中文版),2005,17(1):58-59.