光因子对石刁柏愈伤组织生长过程中蛋白质含量及酶活性变化的影响

EFFECTS OF LIGHT FACTORS ON SOLUBLE PROTEINS AND ENZYMES ACTIVITIES DURING GALLUS GROWTH OF ASPARGUS OFFICINALIS L.

在不同的光因子条件下，石刁柏愈伤组织的生长曲线均呈“Ｓ”型。愈伤组织的可溶性蛋白质含量以黄光最高，其次为蓝光、黑暗、白光、绿光、红光。在蓝光、黄光、红光条件下，愈伤组织的非特异性酯酶活性均出现３个峰，而绿光、白光、黑暗条件下则出现２个峰，以第２７天的峰值相比，其峰值的大小顺序为：黄光、蓝光、白光、红光、绿光、黑暗。除红光为２个过氧化物酶活性峰外，其他均为１个活性峰，其峰值的大小顺序是：黄光、蓝光、绿光、白光、黑暗、红光。各光因子条件下均出现２个超氧物歧化酶活性峰，并以白光条件下的峰值最高。

The callus growth curves of Asparagus officinalis L.presented 'S' types under different light factors but with a similar irradiance.The quantities of soluble proteins of callus were the highest in yellow,in turn in blue,dark,white,green and red lights. The activity of non-specific esterases(NSE)had three peaks in blue, yellow and red lights, but two peaks in green, white and dark lights.The decline of the peak values was in regularorder of yellow,blue, white,red,green, dark lights in the twenty-seventh-day.Except that it had two peaks in red light,the activity of peroxidase(POD)had one peak in the other lights.The decline of the peak values was in regular order of yellow,blue,green,white,dark and red lights.The activity of superoxide dismutase(SOD) presented two peaks in different light factors,and the peak value in white was the highest.