固相萃取同时提取尿中的39种药毒物

Analysis of 39 drugs in urine by HPLC after solid-phase extraction

目的建立固相萃取方法同时提取尿中的39种药毒物并用高效液相色谱法进行分析。方法以多沙普仑为内标,1ml尿样经Oasis小柱固相萃取,用HPLC进行分析。结果39种药毒物可同时从尿中提出,内源性物质不干扰测定。其绝对回收率除吗啡外均大于70％;天内及天间精密度均小于10％;检测限1-15ng／ml;线性相关系数在0.9977以上。结论本法快速、简便、重现性好,空白干扰小,可用于实际案例的药物毒物筛选。

Objective To develop a specific, sensitive, reproducible SPE-HPLC method for simultaneous determination of 39 drugs in urine. Methods With the doxapram as internal standard, Oasis column was used to extract drugs from 1ml of urine. The extracts were analyzed by HPLC. Results 39 drugs could be determined simultaneously in urine, which was not influenced by intrinsic substances in the sample. The absolute extraction recovery rate was>70%. The relative standard deviations for between-day and within-day assay were<10%. The limits of detection ranged between 1ng/ml and 15ng/ml. The detected results showed excellent linearity with the linear correlation coefficient ≥0.9977. Conclusion The method is effective, simple and reliable and may be used in practice.