摘要
The activation energy of the enzyme-catalyzed reaction for uric acid decreases markedly in the presence of o-phenanthroline, which activates the bioelectrochemicla activity of the polypyrrole uricase electrode. The response current of the enzyme electrodeis independent of the concentration of o-phenanthroline. Based on the experimental results, the mechamsm of the enzyme-catalyzed reaction for uric acid in the presence of o-phenanthroline is presented as follows: E+A→EA, EA+S EAS, EAS→EA+P, where E, A, S and P are the enzyme,activator, substrate and product, respectively. The effects of pH value, potential and the uric acid concentration on the response currents of the uricase electrode have been studied in the presence of o-phenanthroline . In the presence of o-phenanthroline , the response current of the enzyme electrode increases linearly with increasmg concentration of uric acid in the region of 0.07 to 0.67 mmol·L-1 , therefore the polypyrrole uricase electrode which has once lost its activity can be activated and used again to determine the substrate concentration.
The activation energy of the enzyme-catalyzed reaction for uric acid decreases markedly in the presence of o-phenanthroline, which activates the bioelectrochemicla activity of the polypyrrole uricase electrode. The response current of the enzyme electrodeis independent of the concentration of o-phenanthroline. Based on the experimental results, the mechamsm of the enzyme-catalyzed reaction for uric acid in the presence of o-phenanthroline is presented as follows: E+A→EA, EA+S EAS, EAS→EA+P, where E, A, S and P are the enzyme,activator, substrate and product, respectively. The effects of pH value, potential and the uric acid concentration on the response currents of the uricase electrode have been studied in the presence of o-phenanthroline . In the presence of o-phenanthroline , the response current of the enzyme electrode increases linearly with increasmg concentration of uric acid in the region of 0.07 to 0.67 mmol·L-1 , therefore the polypyrrole uricase electrode which has once lost its activity can be activated and used again to determine the substrate concentration.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
1994年第7期648-653,共6页
Acta Physico-Chimica Sinica
关键词
催化反应
邻菲罗啉
尿酸酶
酶电极
Uricase electrode, Activator, Kinetics of the enzyme-catalyzed reaction