摘要
用CTAB法直接从食用菌菌丝体培养物中提取 DNA,并将所提取DNA进行PCR,得到了较清晰的扩增图谱,用该法提取的食用菌菌丝体DNA可用于分子生物学研究,具有简便易行的优点。
The DNA of edible fungi was directly extracted from fresh mycelium growing on the solid medium. PCR was performed with total DNA as a template at an annealing temperature of 56℃;Positive amplification was obtained from the mycelium of edible fungi. The result indicated that the method was simple, easy and reliable for DNA extraction from the mycelium of edible fungi cultured on solid medium.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第3期66-68,共3页
Food Science
基金
中国博士后基金(2003033496)
