心房颤动心房组织内血管紧张素原基因表达的研究

Change of gene expression of angiotensinogen and renninin human atria during atrial fibrillation

目的探讨心房颤动(房颤)患者心房组织内血管紧张素原(ATG)和肾素基因的表达。方法将39例风湿性心瓣膜病接受换瓣手术者于术中获取的右心耳(约100mg)分为三组,其中窦性心律组15例,阵发性房颤组8例,持续性房颤组16例(≥6个月),以β-actin为内参照基因,通过半定量逆转录-聚合酶链反应(RT-PCR)技术,测定各组心房组织中ATG及肾素的mRNA含量。结果和窦性心律组相比,ATGmRNA表达在阵发性和持续性房颤组均显著增加,且随着房颤时程的延长而明显增加;在右心房组织中未能检测到肾素基因的明显表达。结论房颤患者心房组织中ATGmRNA表达上调可能与房颤的发生、维持有关,可能是ACEI类药物能防治房颤的机制之一。

Objectives The purpose of this study was to determine the gene expression of angiotensinogen(ATG)and renin in patients with atrial fibrillation (AF). Methods A total of 39 patients with rheumatic heart disease were include.15 patients had no history of AF,8 patients had paroxysmal AF(pAF)and 16 patients with persistent AF(≥6 months PAF).Atrial tissue was obtained from the right atrial appendage during valve replacement surgery.Total RNA was isolated and reversely transcribeb into cDNA.In a semi-quantitative polymerase chain reaction the cDNA of interest genes and of the housekeeping gene β-actin were complified and separated on an agarose gel by electrophoresis and stained with ethidium bromide.The levels of mRNA expression were quantified by densitometry. Results The amount of ATG was increased to 0.61 during PAF(P=0.026)and to 0.78 during cAF(P<0.01)compared to patients with sinus rhythm (0.42).Compared with patients with pAF,the gene expression of ATG in patients with PAF was also increased(P<0.05).The gene expression of renin cannot be detected. Conclusions The up-regulation of ATG mRNA in human atria may have correlation with the initiation or maintenance of AF,and may contribute as a molecular mechanism that the ACEI reduced the incidence of AF.