河蚌外套膜的组织培养

THE TISSUE CULTURE OF THE MANTLE OF FRESHWATER CLAMS

本文报道了褶纹冠蚌和背角无齿蚌外套膜的组织培养结果。试验表明,河蚌外套膜的上皮细胞和结缔组织的成纤维细胞能在离体培养条件下生长繁殖。在试验中还能观察到所培养的组织块上皮细胞,能分泌出茶褐色的有机珍珠质,使组织块逐渐变成茶褐色。

The tissue culture of the mantle of freshwater clams Criaria plicato and Anodontawoodiana elliptica has been carried out. Both clams are generally used in artificialpearl cultivation in our country. A balance salt solution and a sythetic culture mediumhad been designed and improved. Epithelial cells and fibroblast cells can grow andreproduce well in the culture. It has been observed that the epithelial cells do secretedark brown organic pearl matter, which makes the tissue pieces becomes dark brown-coloured.