西洋参悬浮细胞培养条件的研究

A Study on the Optimum Conditions of Cell Suspension Culture of Panax Quinquefolium

通过试验论述了不同愈伤组织年龄和接种量对细胞悬浮培养的影响，从而得出最佳接种年龄及其最佳接种量；分析了培养方式和转达对细胞悬浮培养影响的原因，确定了有利于细胞生长的培养方式及转速；阐述了在美国ＶｉｒＴｉｓ公司生产的ＯＭＮＩ－ＣＵＬＴＵＲＥＢＥＮＣＨ－ＴＯＰ－２Ｌ发酵罐中，进行西洋参细胞发酵培养的结果。描述了西洋参细胞在发酵罐中的生长曲线、培养液中Ｄｏ变化曲线和培养过程中ｐＨ的变化曲线，为西洋参细胞发酵培养条件最佳化的研究提供了有关数据。

The effects of callosity and inocultive volume on the growth rate of the suspeusion ctilturecells of panax quinquefolium are studied and the optimum inoculative age and volume are discovered.To find the optimtim culture means and stirrer rate,the veasous are aualyzed and the above means andrate are proved to affect the growth rate of the suspension culture cells. The dissolved oxygen and pHcurves in the OMNI-OULTURE BENCH-TOP-2L bioreactro(made in the USA)are investigatedwith the suspension cuIture cells of panax quinquefolium,the results of which are valuable for the sus-pension cell cuIture of panax quinquefolium.