甲基睾丸酮诱导鲫鱼雌核发育子代性转化的研究

STUDY ON THE METHYLTESTOSTERONE INDUCTION OF SEX REVERSAL IN GYNOGENETIC PROGENY OF CRUCIAN CARP

本文介绍了用甲基睾丸酮诱导鲫鱼雌核发育子代性转化的研究结果。对孵化后3天的人工雌核发育的红鲫仔鱼和天然雌核发育的银鲫仔鱼,以含有不同量的甲基睾丸酮激素饵料喂养90天,再用一般饵料喂养直到可以剖腹鉴别性别。结果是:1,喂用每克 MT_(25-30μg)激素饵料的,除 1尾发育为精卵巢外.全部试验鱼都发育成雄性;2,喂用每克含 MT_(50-100μg)激素饵料的,没有雄性鱼出现。此外,用 1ml 工业酒精同 1g饵料配制的混合饵料,喂养90天的试验鱼,有60—84.6％的个体发育成雄性。不加激素和酒精的饵料喂养的对照组鱼,全部发育成雌性。

The experiment artificially gynogenetic fries of Carassius auratus auratus (redvariety) and the naturally gynogenetic ones of Carassius auratus gibelio, at the thirdday after hatching, were administered orally with methyltestosterone (MT) at fourdifferent concentrations: 25μg, 30μg, 50μg and 100μg per gram of diet. The dailyhormone diet, at about 7% as weight of the fry, was divided into two parts to feedat a. m. and p. m. This process was continued for 90 days and thereafter,the hormone-treated fry lived on a normal diet until they reached the stage at which sexingwas possible by laparotomy. The results of experiment were as follows: 1. the fries treated, with MT 25μg orMT-30μg hormone diet, developed into males except one having a gonad of ovotestis. 2.no males appeared by treating with MT-50μg or MT-100μg hormone diet. 3.60-84.6%gynogenetic fries of both kind of fish developed into males while they were daily fedwith the diet mixed with 95% crude alcohol (oneml per gram of diet) for a period of90 days. 4. the gynogenetic fry of the both sexes were fed with a normal diet onlythey all developed into females.