摘要
本文报道了4—碘苯氧乙酸(4—IPOAA)在海带夏苗培育中应用的试验结果。4—IPOAA对海带配子体和幼孢子体的生长发育有着明显的影响,高剂量的4—IPOAA(500mg/L)对配子体的发育有明显的抑制作用;解除4—IPOAA对配子体的抑制,配子体在短期内即能正常排卵、排精并受精形成孢子体;低浓度的4—IPOAA(25mg/L以下)能促进幼孢子体的生长。这项试验结果表明,有可能利用4—IPOAA对配子体的抑制特性,来改进目前所采用的低温、自然光条件下培育海带夏苗的工艺。作者建议把海带夏苗培育工艺改为以下步骤:(1)在常温下采苗;(2)在18℃以下水温条件下培育配子体;(3)在配子体成熟前用4—IPOAA处理,使其停止发育,在较高水温(20℃左右)条件下静水培养被抑制的配子体;(4)在计划夏苗出库前40—50天,解除4—IPOAA对配子体的抑制,恢复最适条件下流水培育;(5)肉眼见苗后及时下海暂养。
This paper is based on the result of preliminary study on the application of 4-IPOAA to culture summer seedlings of Laminaria, for the purpose of improving the technique of summer seedlings. The experiments were carried out three times in 1979-1981.The common method, in rearing the summer seedlings is usually by lowering temperatures, natural sunlight exposure and running seawater in a green house, from June to October the natural temperature of the seawater are not suitable for the growth of Laminaria, the atove method would take about 80-150 days for the rearing of summer seedlings in green house. It it also rather expensive.This study-wasmade to devise a new method in improving the technique. The main problem in the new method was to find a suitable concentration of 4-IPOAA in order to inhibit the development of the gametophytes for a certain period.The result of the experiment are summarized in following steps;1.to collet zoospores under ordinary temperature;2.to rear gametophytes undeu lower temperature for several days;3.to treat gametophytes with 4-IPOAA before the formation of gametes;4.to stop the treatment with 4-IPOAA and culture gametophytes under optimal conditions;5.to culture seedlings about 50 days;The seedlings thus reared was found to be very healthy and grow normally. A large scale experiment will be carried out to find the posibility of using this method for cultivation industry.
