菲律宾蛤仔室内催产研究——阴干、氨海水和性诱导法

EXPERIMENTS ON IN-DOOR INDUCED BREEDING OF RUDITAPES PHILIPPINARUM

本文初步探索菲律宾蛤仔在室内催产方法和步骤:即先将性腺成熟的亲蛤阴干、后用氨海水浸泡,再以氨海水加雌性腺稀释液诱导亲蛤排放精卵。 试验结果是:阴干时间1/2至24小时都可以催产,一般应控制在16小时内。氨海水催产有效浓度为0.00015N至0.00225N,以0.00075N氨海水,浸泡14小时效果最好。0.00075N氨海水加雌性腺稀释液(适宜浓度为25至150毫克/升)联合诱导,催产成功率达96.2％。 用本文催产方法特点是:潜伏期短,一般在30分钟内;催产率高,在海区产卵高峰期,室内催产率72-100％;排卵量大,多数在76万粒/个以上;受精率、孵化率和D形面盘幼体出现率均接近100％,并能在室内条件下正常发育变态,培育到稚贝。

The method and steps of in-door indueed breeding of Huditapes Philippinarum is discribed in this paper: The sex-matured parent elams should be air-dried for a period and then soaked in ammonia sea water by adding with diluted solution of famale sexual glannds for inducing spawingThe results obtained from the experiments are as follows: Spawning could be successful with 1/2—24 hours of air-drying. Generally, the optimun period of airdrying should be controlled within 16-hours. The range of effective concentration-of ammonia and seawater for inducing was 0.00015—0.00225N. The best results are obtained from the parent clams soaked in 0.00075N of ammonia and seawater with addition of female sexual gland solution at a concentration of 25—150mg/L for 14 hours. The sucess rate reaches to 96.2％.The characteristics for the method described above are: short cryptic period, usually within 30 minutes; high spawning rate about 72—100％ during the natural spawning season high festility, The rates of fertilization, incubation and the development of larvae into the viliger stage nearly reach to 100％ and they can normally develop into spats under in-door conditions.