用分子杂交法检测妇女宫颈疾患中的HPVDNA

IDENTIFICATION OF HPV DNA WITH MOLECULAR HYBRIDIZATION FROM THE PATIENTS WITH GENITAL DISEASES

为了探讨人乳头瘤病毒（ＨＰＶ）与宫颈癌之间的相关性，我们收集了全国宫颈癌高发县之一的略阳县１２例宫颈癌，１２例宫颈间变，３６例宫颈Ⅱ～Ⅲ度糜烂患者的组织标本，经抽提ＤＮＡ以后，以［ａ－３２Ｐ］ｄＡＴＰ标记ＨＰＶ１６、１８型ＤＮＡ进行打点杂交。结果为宫颈癌ＨＰＶ１６阳性６例，ＨＰＶ１８阳性１例。宫颈间变ＨＰＶ１６阳性４例，ＨＰＶ１８阳性１例。ＨＰＶ１６阳性率分别为５０％和３３．３％，ＨＰＶ１８阳性率为８．３％。宫颈糜烂Ⅱ～Ⅲ度的患者组织ＤＮＡ与ＨＰＶ１６、ＨＰＶ１８混合杂交阳性１７例，阳性率为４７．２％。

in order to study the relationship between human papillomaviruses (HPV) and cervical cancer, 12 biopsies of cervical cancer, 12 biopsies of cervical intraephithial neoplasia and 36 smears of cervical erosion(grade Ⅱ ￣ Ⅲ) were collected from Lueyang County, Shaanxi Province, one of the high incidence areas of cervical cancer in China. Extracted tissue DNAs were hybridized with [a-32P) dATPlabelled HPV-16 and HPV-18 DNAs. The results showed that the positive rates of HPV-16 and HPV18 were 50/00 (6112i and 8. 3/00 (1/12) respectively In the group of cervical cancer, 33. 3/00 (4112) and 8. 3/00(1/12) in the group of CIN. In the group of cervical erosion, 17 cases of tissue DNAs were hybridized with.mixed HPV-16 and HPV-18 DNA probe ' the positive rate was 47. 2/00 t17136 ).