摘要
在注射低剂量LRH-A的同时注射多巴胺拮抗物pimozide(PIM),能显著增强LRH-A诱导的排卵效应,排卵率达到100％。 LRH-A+PIM能促进脑垂体GtH细胞的合成和分泌,而注射多巴胺能抑制LRH-A诱导的GtH释放活动。
A single injection of low dose LRH-A alone was relatively ineffective for inducing ovulation in Paramisgurnus dabryanus, however, the injection of low dose LRH-A in association with pimozide (PIM), a dopamine antagonist, significantly potentiated the effects of LRH-A on ovulation, the rate of ovulation being 100%. The histological and ultrastructural studies of pituitary showed that the injection of low dose LRH-A together with PIM greatly potentiated the LRH-A stimulated synthesis and release of GtH, whereas the injection of dopamine (DA) blocked the stimulation of GtH release induced by injection of LRH-A. These results demonstrated that dopamine, having GtH release iuhibitory activity in Paramisgurnus dabryanus, acts directly at the pituitary level, so, the combination of treatment with PIM and LRH-A is a highly effective means of inducing ovulation in this fish.
