从西安地区正常儿童尿中检测人巨细胞病毒DNA

SURVEY OF URINE SPECIMENTS FROM KINDERGARTEN CHILDREN IN XI'AN AREAS FOR CYTOMEGALOVIRUS

为了研究人巨细胞病毒（ＨＣＭＶ）感染的流行病学及鉴定ＨＣＭＶ核酸在尿中分布，我们用ＨＣＭＶＡＤ１６９株ＨｉｎｄⅢ－ＥＤＮＡ片段制备探针，采用核酸杂交技术对取自西安地区１８０例３岁～５岁正常儿童的尿标本进行了ＨＣＭＶ相关序列的检测。结果显示出西安地区ＨＣＭＶＤＮＡ阳性者３例（５０份尿标本）占６％；咸阳地区ＨＣＭＶＤＮＡ阳性者７例（５０份尿标本）占１４％；兴平地区ＨＣＭＶＤＮＡ阳性者１９例（８０份尿标本）占２５％。在不同的地区之间，阳性检出率有较明显地差异。

In order to study the epidemiology of Human Cytomegalovirus(HCMV) infection and to identify the presence of HCMV DNA in urine,the specific mucleic acid probe was prepared which contains the Hind Ⅲ-E DNA fragment of HCMV strain AD 169. The 180 urine specimens of 3～5 aged kindergarten children, in Xi'an areas were detected by nucleic acid hybridization. Results showed that the positive rate of HCMV DNA was6%(3/50) in Xi'an 14%(7/50)in Xianyang and 25%(19/80) in Xinping. Defferent positive rate among the areas was observed.