反相高效液相色谱中溶质的保留机理与能量守恒

Retention Mechanisni in Reversed Phase LiquidChromatography and Conservation of Energy

由于在反相液相色谱（ＲＰＬＣ）中的分配保留机理忽略了在溶质进入固定相时溶剂分子必须要离开固定相的这一事实，所以，首次得出了ＲＰＬＣ中的分配机理是违背了物理学中的两个基本原理，一个空间不能同时被两个物体占据和能量守恒定律的结论。分配机理不考虑固定相对溶质保留的贡献和惯常置换（吸附）机理不考虑流动相对溶质保留的贡献是不合理的。溶质的计量置换保留模型（ＳＤＭ—Ｒ）不仅符合上述的两个物理学基本原理，而且考虑了ＲＰＬＣ体系中分子间的各种相互作用。用文献中发表的本是用来支持分配机理的原始ｋ’值，着重检验和比较该ＳＤＭ—Ｒ与分配机理的结果，发现这些数据更支持ＳＤＭ—Ｒ。还得出上述结论亦适用于生物大分子。首次证实ＳＤＭ—Ｒ可以用来统一迄今仍在争论的ＲＰＬＣ中的分配机理和置换机理。

Owing to ignoring the fact that the solvent molecules necessarily leave away from station-ary phase as solute molecules entering the stationary phase for the partition retention mechanism in re-versed-phase liquid chromatography (RPLC) ,it was firstly concluded that the partition mechanism inRPLC is against the two physically fundamendal pricinples , conservation of energy and one space cannot be occupied by two bodies in a same time. Without taking account into the contributions of thestationary phase to the retention of solute in partition mechanism and that of mobile phase to it in tra-ditional displacement (adsorption) mechanism in RPLC, both are unreasonable. Stoichiometric dis-placement model for retention (SDM-R) of solute was concluded that it is not only following the twophysical principles pointed above, but also taking account in each kinds of molecu[ar interactions oc-curing in RPLC system. The results of SDM-R and the partition mechanism are specially tested andcompared. It was found that these k' data are more supporting the SDM-R than the partition mecha-nism. The foregoing conclusion is also validity for biopolymers. Ths SDMR was firstly proved that itcan be used to unify both partition and displacement retention mechanismi in RPLC which have notbeen solved yet.