鲤鱼不同品种(系)红血球抗原特异性的初步研究

PRELIMINARY STUDY ON THE SPECIFICITY OF RED BLOOD CELL ANTIGENS IN VARIOUS VARIETIES (STRAINS) OF CARP

用血球凝集和血清吸收试验的方法,对若干个鲤鱼品种及鲤鱼人工雌核发育系的红血球中的某些同种异型抗原纯合性进行了初步研究。①以抗雌核发育红鲤抗血清(abs/R)为试剂,三个雌核发育系(R_(8305),R_(8413),RMGF_1)的RBC反应值分别为1521—2360,1521—2028,853—1521,普通红鲤、镜鲤、白鲢的反应值在80—782。②abs/8305与abs/8413经靶细胞相互吸收后的残留活性显著降低(均为320),而以自鲢RBC吸收后抗血清活性不变。作者认为这部分抗原在鲤鱼种以下的品种和品系中具有特异性,可考虑将其作为鱼类品系鉴定的一种手段。

This paper reports the results of preliminary study on the specificity of red blood cell antigens from varieties (or strains) of carp (Cyprinus carpio L.). By means of hemagglutination and serological absorption tests, cross reactions between special antiserum and RBC of gynogenetic red carp F_2 and gynogenetic red mirror carp F_1 (RMGF_1) were investigated. It was found that the reaction values are different between these strains, but the values of random samples in a strain are of the same level. These results show that the genes controlling the red blood cell allotypic antigens of carp are homogenous to a great degree in the same strain. Among these tested strains, R_(8305) and R_(8413) have the most close reaction values and their absorptions are also identical. It implies that R_(8305) and R_(8413) are very similar in their genotypes. The differences among three gynogenetic strains (R_(8305), R_(8413) and RMGF_1) and normal red carp_2 mirror carp and silver carp (Hypophthalmichthys molitrix) are quite obvious. It appears that the allotypic antigens of RBC are peculiar to given carp strain or variety, and it can be considered as one of the markers for fish strain identification.