快生型大豆根瘤菌生物学特性研究

Biological Characteristics of Four Fast-Growing Rhizobium Japonicum Strains

由当地分离纯化并回接有效而获得的４株快生型大豆根瘤菌株，通过形态、生理鉴定，表明与慢生型菌株有明显差异；在菌株与大豆品种亲和性的盆栽试验中，其中Ａ０３４菌株与“徐州四号”、Ａ０３２与“东解选”大豆品种间具有良好共生效应；其株高、茎叶干重及含氮量、株瘤数与瘤重、及固氮酶活性等，均明显高于不接种对照；固氮率分别提高为４８．１％与４４．５％；

Four fast-growhg Rhizobium japonicum strains were isolated,purifiedand back-nodulated to soybeans in the local areas.The results from the identification ofmorphology and physiology showed that there were obvious differences between thesestrains and slow-growing Rhizobium japonicum strains.In the pot experiments of anffinity between the strains and soybean cultivars,the excellent symbiotic effect wasfound among strain A034 and soybean cultivar-Xuzhou NO.4 and strain A032 and soy-bean cultivar-Dong Xiexuan,whose plant height,dry weight and nitrogen contents ofstems and leaves,and also activity of nitrogenase were higher than those of the controlsoybean plants(without inoculation).Nitrogen-fixing efficiency was increased by 48.1%and 44.5%respectively.