奶山羊冻胚长期保存长途运输和移植试验

Long-term Cryopreservation,Long-distance Transportation and Transfer of Dairy Goat Embryos

用ＲＰＥ简易冷冻器快速冷冻６～７日龄萨能奶山羊胚胎。将在液氮中冻存１２～８１９ｄ的胚胎用液氮罐经汽车或火车运至外地，并把解冻后的７３枚胚胎移植给３８只当地山羊。受体妊娠率为６０．５％，移植胚产羔率为３９．７％。胚胎冻存１２～２１，１９８～２６５，３２０～４８０和６８７～８１９ｄ时，移植产羔率分别为３８．５％，４５．８％，４１．７％和２５．０％。各冻存期间差异不显著（Ｐ＞０．０５）．

Embryos from superovulated Saanen dairy goats on days 6-7(matingday=day 0)were quickly frozen in a simple RPE freezer. After being frozen for 12～819days,a part of embryos was transported by car or train to other places of the countryusing a jar with LN_2.After thawing,73 embryos were transferred into 38 recipients ofindigenous goats. Pregnant rate of recipients was 60.5%(23/28).Kidding rate of thetransferred embryos was 39.7%(29 /73).After being frozen for 12～21, 198～125,320～480 and 687～819 days,no significant difference(P>0.05)was found in kiddingrates of transferred embryos which had been frozen for different duration.