摘要
单细胞绿藻的最适生长温度,一般都在25℃上下,这给许多试验工作,特别是夏季露天浅层培养和室内高光强强化培养工作造成了大幅度降温的额外负担。而高温品系则可以减轻降温的困难,并且具有高产的特征。本文报道我们培育栅藻高温品系的结果。观察了在长期高温(暗期36℃,光照期42℃)培育后细胞形态上的变化,并着重研究了生长繁殖规律,获得了同步培养。细胞生长在光照下进行,细胞分裂在暗中进行,光照12小时后细胞成熟,光照停止后4小时内,出现孢子形成和子细胞释放的高峰。在2,400 lx 的弱光照条件下,生长率K 值仍可达到2.07(以每日log_e 单位计),每个母细胞平均可产生8.7个子细胞,显示了生长繁殖迅速的特点。
A high-temperature strain of green alga Scenedesmus obliquus selected by incu-bating the cells under the high temperature condition for a long period has beenobtained.The morphological and developmental changes of cells after a long periodof high temperature cultivation (36℃ in the dark period,42℃ in the light period)have been observed,and a synchronous culture has been obtained.The growth ofcells occurs under illumination and the cell division takes place in the dark.Twelvehours after the beginning of the light period,cells become ripening,and transforminto“mother cells”.The production of the autospores and the maximum autosporeliberation set in within four hours after the beginning of the dark period.Theaverage number of daughter cell produced is 8.7.The growth rate K is up to 2.07(log_e units per day) under unsaturated light intensity.This high-temperature strainis characterized by its rapid growth.
