摘要
从分布于甘肃省河西走廊的沼泽芦苇和沙丘芦苇叶中提纯的Rubisco,大、小亚基分子量无明显变化,但沙丘芦苇与沼泽芦苇相比较,Rubisco的氨基酸组成中,亲水性氨基酸相对含量增加,疏水性氨基酸相对含量减少,酶分子的疏水性降低,且酶分子中可被PCMB滴定的SH基数仅为沼泽芦苇的50%。这说明,芦苇在长期适应沙丘干旱生境过程中,其Rubisco无论在结构与功能上,均发生有适应性的改变。
There was no change on molccular weight of small and large subunits of Rubisco purifiedfrom Swamp-reed and Sand dune-reed(Phragmites communis)distributed in the Hexi Corri-dor, Gansu Province. There was different amino acid composition in Rubisco purified from differ-ent habitat reeds plant. Drought stress induce a higher hydrophility and a lower average hy-drophobicity of Rubisco. The Rubisco purified from Sand dune-reed plants contained about 50%of titratable SH groups as compared with that the Swamp-reed plants. The results indicate thatSand dune-reed plants,drought induces conformational changes and gene expression changes inRubisco which may be responsible for its adaptation to drought stress,it also indicates that it issuitable to conside the Swamp-reed and Sand dune-reed as two ecotypes.
出处
《西北植物学报》
CAS
CSCD
北大核心
1994年第3期193-197,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金
关键词
芦苇
RUBISCO
干旱
生境
Different habitat reed , amino acid composition , subunits,hydrophobicity,SH group