百合花药壁层的发育及组织化学研究

DEVELOPMENT AND HISTOCHEMICAL OBSERVATIONS ON ANTHER WALL LAYERS OF LILIUM BROWNII F.E.EX MIELLEZ VAR.VIRIDULUM BAKER

对生长在陕西留坝的百合（ＬｉｌｉｕｍｂｒｏｗｎｉｉＦ．Ｅ．ｅｘＭｉｅｌｌｅｚｖａｒｖｉｒｉｄｕｌｕｍＢａｋｅｒ）的花药壁层发育过程，特别是绒毡层的发育做了形态学观察。其结果是：百合花药壁层的发育方式为基本型。花药绒毡层属腺质绒毡层类型。在单细胞花粉阶段后期，部分花粉粒壁一侧凹陷时，绒毡层细胞内切向面上出现乌氏体。随着发育阶段的推移，乌氏体的数量有所增加。在光学显微镜下观察，每个乌氏体只有一个乌氏体芯。在乌氏体出现时，也可观察到花粉外壁外层的出现。到二细胞花粉时，花药开裂之前，绒毡层细胞解体消失。本文同时应用组织化学技术，对壁层发育过程中多糖类以及蛋白质等的动态也进行了观察。

The development of the anther wall,special of the tapetum,in lilium brownii F.E.exMiellez var. viridulum Baker,which living in LiuBa County,were studied morphologically. Theontogeny of anther wall layers belong to basic type. The development of tapetum is glandular type. At unicellilar stage, Ubischbodies appear on the inner tangential face of the tapetum whensome pollen involuted laterally. With the development of anther, Ubisch bodies may becomelarger and more, and there is only one core in each of them. At bicellular stager, tapetal cells de-generate and leave over a few of Ubisch bodies in the theca. In this paper,some histochemicaltechnique are also used to observe the development of the starch grains and accutnulated proteinin the process of wall layers ontogeny.