离体背根神经节细胞膜电学特性的测量及其应用

Measurement of Electrical Characteristics of Somatic Membrane and Its Application in Dorsal Root Ganglion Neurone in Vitro

本文介绍了蟾蜍及新生大鼠离体背根神经节神经细胞膜电位及诱发动作电位的细胞内记录方法以及膜电导,翻转电位等电学特性的测量。应用上述方法测得蟾蜍DRG细胞膜输入电阻为30～40MΩ,新生大鼠的输入膜电阻为45～70MΩ;α_1肾上腺素能受体激活引起DRG细胞膜去极化反应时,膜电导减小.而α_2-肾上腺素能受体激活引起细胞膜电位超极化反应时,膜电导增加。去甲肾上腺素引起DRG细胞膜去极化反应的平均翻转电位值为-88.5mV。这些方法为神经细胞的某些电学特性、神经递质及受体等方面的研究提供了基本的实验手段。

The method of intracellular recording of neural cell membrane potential and evoked action potential in isolated toad or new-born rat dorsal root ga-nglion(DRG)was introduced. In addition, measurement of membrane conductance and reversal potential was described as well. We observed that the membrane input resistances were found to be 30-40 Ω in toad DRG and 45-70Ω in new-born rat DRG respectively,by means of the above methor. The depolarization response of DRG cells membrane induced by α1-adrenoceptors activation was associated with a decrease of membrane conductance.and the hyperpolarization response induced by α2-adrenoceptors activation was accompanied by an increase of membrane conductance. The mean reversal potential of noradrenaline-induced depolarization responses was -88.5 mV. The method introduced in the present paper is a valuable way of investigating some electrical characteristics, neurotransmitters and receptors of neural somatic membrane.