慢性肝病患者血清和肝组织前S蛋白的表达及其相互关系

The Relation of Pre-S in Serum to Pre-S in Liver in Patients with Chronic Liver Diseases

采用本室建立的标记抗生物素-生物素酶联免疫试验及间接酶免疫试验进行血清与肝组织前S蛋白检测。慢性乙肝病毒感染者肝组织前S_1和前S_2的检出率略高于血清标本;血清前S_1和前S_2的相对含量与同一患者肝内前S_1前S_2阳性细胞数有一定正相关关系,但与前S_1、前S_2表达方式及其肝脏病理变化无明显关系。

Labelled avidin-biotin-ELISA (LAB-ELISA) was established to detect pre-S proteins in serum, and it is verified that specificity of this technique is fairly high as compared with control experiments. It was found in our study that the incidence of pre-S proteins in liver is slightiy higher than that in serum in patients with chronic HBV infection and the relative amount of both pre-S1 and pre-S2 in serum is in some degree associated with the number of positive cells for pre-S1 and pre-S2 in liver. However it is not significantly associated with expressive patternsof pre-S1 and pre-S2 and pathological changes in liver.