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利用EST数据库资源克隆家蚕Bombyx mori6-磷酸葡萄糖酸脱氢酶基因 被引量：4

Cloning 6-phosphogluconate Dehydrogenase(6PGDH)of the Silkworm Bombyx mori by Selecting EST Database
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摘要 以果蝇6-磷酸葡萄糖酸脱氢酶(6-phosphogluconatedehydrogenase,6PGDH)基因cDNA序列为信息探针,对家蚕EST数据进行同源检索筛选,克隆到了家蚕6-磷酸葡萄糖酸脱氢酶基因的cD鄄NA序列,该基因全长2011bp。经RT-PCR克隆、序列分析验证,结果表明与电子克隆序列完全一致;该基因具有完整的开放阅读框(ORF),编码蛋白为483个氨基酸;通过与人、果蝇、家鼠、摇蚊、线虫的6PGDH蛋白序列比较,发现该基因具有高度的保守性。 A novel silkworm(Bombyx mori) 6-phosphogluconate dehydrogenase (6PGDH) gene was cloned by blasting its EST database with the fruit fly homologous 6PGDH gene cDNA sequence information as a querying probe. It was also verified by RT-PCR. The full-length 6PGDH of 2011bp contains a complete open reading fragment (ORF: 117-1566) and encodes 248 Aa protein, which was very conservative in Homo sapiens, Drosophila melanogaster, Anopheles gambiae, Mus musculus, Caenorhabditis elegans.
作者 沈卫锋 牛宝龙 翁宏飚 孟智启
机构地区 浙江省农业科学院蚕桑研究所
出处 《蚕桑通报》 2005年第1期14-19,共6页 Bulletin of Sericulture
基金 国家自然科学No:30371087。
关键词 EST数据库 RT—PCR 6-磷酸葡萄糖酸脱氢酶基因 家蚕 ESTdatabase RT-PCR 6-phosphogluconate dehydrogenase Bombyx mori
分类号 S881 [农业科学—特种经济动物饲养]
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参考文献9

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二级参考文献34

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