实验性抗基底膜肾小球肾炎——免疫荧光、光镜和电镜研究

Immunofluorescent, Light and Electron Microscopic Studies on Experimental Antiglomerular Basement Membrane Glomerulonephritis

采用自制兔抗大鼠肾免疫血清,制造大鼠抗基底膜肾小球肾炎模型,并进行了重复试验。用免疫荧光技术、光镜和电镜进行观察,前后两批实验结果相同,提示在肾小球基底膜上显示出典型的光滑线型荧光图象,在电镜下肾小球毛细血管丛基底膜呈现局灶性电子透明区而疏松增厚,光镜下主要表现为增生性肾小球肾炎变化,未见新月体形成。实验结果可与人类某些肾炎相比较,对了解人类某些类型肾炎的病因、发病机理、病理变化、临床表现及其防治有一定意义。

The experimental antiglomerular basement membrane glomeruloneph-ritis in rats has been induced by on intravenous injection of rabbits anti-rat kidney serum in repeated experiments. The pathological changes of the kidneys were observed by immunofluorescent, light and electron microscopic studies. The results of repeated experiments conformed with each other. Immunofluorescence revealed typical IgG linear fluorescence along glomeruler basement membrane; electron microscopy demonstrated that the basement membrane of glomerular capillary plexus had local eletron-lucent areas and loosely thickened; light microscopy showed proliferative glomerulo-nephritis without the crescent. The experimental results show that experimental glo-merulonephritis is similar to the some kinds of human glomerulonephritis and has, therefore, a certain significance in the detection of etiology, pathogenesis, pathological changes and clinical manifestations of glomerulonephritis and in its prevention and treatment.